Research interests:

Non-B DNA, Aging, Bioinformatics and Genomics, Molecular Evolution, Gene Therapies, Precision Medicine.

Education:

  • Bachelor of Science. Biology – Biochemistry. University of Nantes. France. June 2010
  • Master of Science: Biology. University of Puerto Rico, Mayagüez. June 2013
  • Today: Ph.D. candidate. Bioinformatics and Genomics. Penn State University – Makova Lab

Oral Presentations:

  • Non-B DNA Structures Affect Replication Speed, Sequencing Errors, and Mutation Rates. Oakland University Genomics Symposium. Oakland University – Rochester, Michigan. 2019.

  • Non-B DNA structures affect polymerization fidelity. Lightning talk - Bioinformatics and Genomics Retreat. Pennsylvania State - University Park, Pennsylvania. 2018.

  • Non-B DNA Structures Affect Error and Mutation Rates. Cancer Research Day. Pennsylvania State - University Park, Pennsylvania. 2018.

  • A new dimension to DNA sequencing: polymerization kinetics at non-B DNA structures. Workshop on emerging methods for sequence analysis. Pennsylvania State - University Park, Pennsylvania. 2018.

  • Local context in DNA sequence influences the time between incorporation of consecutive bases. Bioinformatics and Genomics Retreat. Pennsylvania State- University Park, Pennsylvania. 2016.

  • Local context in DNA sequence influences the time between incorporation of consecutive bases. FASEB - Dynamic DNA Structures in Biology. Saxtons River, Vermont. 2016.

Awards

  • Computation, Bioinformatics, and Statistics (CBIOS) Fellowship - Pennsylvania State University. 2018
  • Huck Dissertation Research Grant - Pennsylvania State University. 2015
  • University Graduate Fellowship - Pennsylvania State University. 2013

Publications

  • Long-read sequencing technology indicates genome-wide effects of non-B DNA on polymerization speed and error rate. Guiblet, W.G, Cremona, M.A. et al., Genome Research. 2018.

  • Streamlined analysis of duplex sequencing data with Du Novo. Stoler, N., Arbeithuber, B. et al., Genome Biology. 2016.

  • Sequencing rare and common APOL1 coding variants to determine kidney disease risk. Limou, S. et al., Kidney Int. 2015.

  • Drosophila Muller F Elements Maintain a Distinct Set of Genomic Properties Over 40 Million Years of Evolution. Leung, W. et al., G3. 2015.

  • SmileFinder: a resampling-based approach to evaluate signatures of selection from genome-wide sets of matching allele frequency data in two or more diploid populations. Guiblet, W.M. et al., GigaScience, 2015.

  • Reconstructing Native American Migrations from Whole Genome and Whole-Exome Data. Gravel, S. et al., Plos Genetics. 2013.

  • A locally funded Puerto Rican parrot (Amazona vittata) genome sequencing project increases avian data and advances young researcher education. Oleksyk, T.K. et al., GigaScience, 2012.

Skills:

  • Bioinformatics and genomics, Statistics, Data visualization, Molecular biology
  • Linux / Windows / Mac
  • Python, R, bash
  • Github, Conda,
  • Bedtools, UCSC Genome Browser, Galaxy, Integrative Genomics Viewer, Geneious, MEGA

Event Organization & Student Leadership

  • Secretary of the GenoMix club at Penn State. 2019-2020
  • President of the GenoMix club at Penn State. 2016-2018
  • Organizer at the BioInformatics and Genomics welcome weekend at Penn State. 2017
  • Organizer at the BioInformatics and Genomics Retreat at Penn State. 2014
  • Volunteer at the Annual Meeting of the Society for Molecular Evolution and Biology. 2014
  • Puerto Rican Parrot Conservation crowdfunding events (Save the parrot with style, March and May 2013, Rincón, Puerto Rico; Pasarella, San Juan, Puerto Rico; …)

Teaching assistantships

  • Bootcamp on Reproducible Research. Penn State. 2018
  • Molecular Evolution. Penn State. 2017
  • Populations and Communities – Laboratory. Penn State. 2016
  • Genomics. Penn State. 2014
  • Genetics – Laboratory. University of Puerto Rico, Mayagüez. 2011-2012
  • Biological Sciences – Laboratory. University of Puerto Rico, Mayagüez. 2012-2013

Spoken Languages

  • French (native)
  • English (fluent)
  • Spanish (fluent)

Posters & Others

  • Non-B DNA affects polymerization speed and error rate in sequencers and living cells. Guiblet, W.M., Cremona, M.A., M. Cechova, R. S. Harris, I. Kejnovská, E. Kejonosky, K. Eckert, F. Chiaromonte, K. D. Makova. In: Evolution. Montpellier, France. 2018. Poster, P-0394.

  • Non-B DNA motifs affect polymerization kinetics, errors, and mutation rates. Guiblet, W.M., Cremona, M.A., M. Cechova, R. S. Harris, I. Kejnovská, E. Kejonosky, K. Eckert, F. Chiaromonte, K. D. Makova. In: Population, Evolutionary and Quantitative Genetics (PEQG). Madison, Wisconsin. 2018. Poster.

  • Polymerization kinetics deciphered using PacBio sequencing: Non-B DNA affects polymerase progression and error rate. W.M., Cremona, M.A., M. Cechova, R. S. Harris, I. Kejnovská, E. Kejonosky, K. Eckert, F. Chiaromonte, K. D. Makova. In: The Biology of Genomes. Cold Spring Harbor Laboratory. New York. 2017. Poster.

  • Local context of DNA sequence influences the tempo of base incorporation. Guiblet, W.M., Cremona, M.A., F. Chiaromonte, K. D. Makova. In: FASEB - Dynamic DNA Structures in Biology. Saxtons River, Vermont. 2016. Poster, 8. Signatures of selection surrounding large insertions and deletions in coding regions identified in the hominid lineage genome-wide. Guiblet, W., K. Zhao K., D. Ferrer-Torres, C. T. Ruiz-Rodriguez, A. L. Roca, S. E. Massey., J. C. Martinez-Cruzado, T. K. Oleksyk. In: Annual Meeting of the Society for Molecular Evolution and Biology. San Juan, Puerto Rico. 2014. Poster, 2123.

  • Reconstructing the history of Indigenous American populations from the Thousand Genomes Project admixed populations of the Americas. Zakharia, F., M. Muzzio, K. Sandoval, A. Moreno-Estrada, S. Gravel, J. Byrnes, C. R. Guignoux, J. L. Rodriguez-Flores, B. Maples, W. Guiblet, J. Dutil, E. E. Kenny, A. W. Bigham, M. D. Shriver, The 1000 Genomes Consortium, A. Ruiz-Linares, T. K. Oleksyk, J. C. Martinez-Cruzado, E. Gonzalez Burchard, C. D. Bustamante. In: Annual Meeting of the American Society of Human Genetics. San Francisco, San Francisco, CA. 2012. Poster, 3380W.

  • Signatures of selection surrounding large insertions and deletions in coding regions identified between modern and Denisovan humans, as well as four other species of primates genome-wide. Guiblet, W., K. Zhao, D. Ferrer-Torres, C. T. Ruiz-Rodriguez, A. Roca, S. Massey, J. C. Martinez-Cruzado, and T.K. Oleksyk. In: Annual Meeting of the American Society of Human Genetics. San Francisco, CA. 2012. Poster, 3254W.

  • The Puerto Rican parrot genome project: an international effort supported by the local community. Oleksyk, T.K., J.F. Pombert, D. Siu, A. Mazo-Vargas, B. Ramos, W. Guiblet, Y. Afanador, C.T. Ruiz-Rodriguez, M.L. Nickerson, M. Dean, D. M. Logue, L. Figueroa, R. Valentin, J.L. Rodriguez-Flores, S.E. Massey, J.C. Martinez-Cruzado. In: Society for Molecular Biology & Evolution. Dublin, Ireland. 2012. Poster, P-1555.

  • Search for selection in coding regions surrounding large insertions and deletions between humans and four primate species. Guiblet, W., K. Zhao, J. C. Martinez-Cruzado, S. Massey, A. L. Roca, N. Volfovsky, T. K. Oleksyk. In: Biology of Genomes. Cold Spring Harbor Laboratory, NY. 2012. Poster.

  • Genomic Reconstruction of an Extinct Population from Next-Generation Sequence Data - Insights from the Taìno Genome Project. Byrnes, J. K., J. L. Rodríguez-Flores, A. Moreno-Estrada, C. R. Gignoux, S. Gravel, W. Guiblet, F. Zakharia, J. Dutil, E. G. Buchard, T. K. Oleksyk, J. C. Martínez-Cruzado, C. D. Bustamante, The 1000 Genomes Project Consortium. In: Annual Meeting of the American Society of Human Genetics. Montreal, Canada. 2011. Platform, 104.

  • Search for selection in coding regions surrounding large insertions and deletions between humans and four primate species. Guiblet, W., K. Zhao, A. L. Roca, J. C. Martinez-Cruzado, S. Massey, T. K. Oleksyk. In: Annual Meeting of the American Society of Human Genetics. Montreal, Canada. 2011. Poster, 475 T.

  • Worldwide distribution of Kidney susceptibility alleles and haplotypes around MYH9 gene. Oleksyk, T.K., Nelson, GW, An, P., Guiblet, W., Kopp, JB, and Winkler, CA. In: Annual Meeting of the American Society of Human Genetics. Washington, DC. 2010. Poster.